Structure Bonding (Berlin) 17:67–134Ĭurtis AT, Hori M, Green JM, Wolfgang WJ, Hiruma K, Riddiford LM (1984) Ecdysteroid regulation of the onset of cuticular melanization in allatectomized and black mutant Manduca sexta larvae. Ann Entomol Soc Am 69:365–373Ĭrichton RR (1973) Ferritin. Gen Comp Endocrinol 47:308–322īell RA, Joachim FG (1976) Techniques for rearing laboratory colonies of tobacco hornworms and pink bollworms. J Biochem (Tokyo) 65:915–923īeckage NE, Riddiford LM (1982) Effects of parasitism by Apanteles congregatus on the endocrine physiology of the tobacco hornworm, Mandyca sexta. J Biol Chem 253:4451–4458īaba A (1969) Crystallization of ferritin from coelomics fluid of Corbicula sandai with ammonium sulfate. J Biol Chem 249:634–644Īrosio P, Adelman TG, Drysdale JW (1978) On ferritin heterogeneity. These studies demonstrate the presence of both a transport protein and a unique circulating ferritin in Manduca sexta, the latter serving a storage function during development and possibly also a transport function.Īmes GF (1974) Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Its chemical and ultrastructural characteristics were those of ferritin.
The second protein had a molecular weight of 490 kD with subunits of 24 and 26 kD and contained 220 μg of iron/mg protein. Therefore, it appeared to serve a transport function. This protein bound ionic iron in vitro and was able to transfer this iron to ferritin when incubated with fat body in vitro. The first was composed of a single polypeptide chain of 80 kD, containing one atom of iron. Studies of the hemolymph disclosed two ironcontaining proteins. In the adult a significant portion of this iron was found in flight muscle. By pupation 95% of the labelled iron was found in the fat body.
When 59Fe was fed to 5th instar larvae, it was evenly distributed among the hemolymph, gut and carcass until the cessation of feeding. Manduca sexta larvae accumulate large amounts of iron during their larval feeding period.
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